A Longitudinal Study of NADC34-Like Strains in an Intensive Farm Unravels Divergent Evolution

Figure 3

Recombination and phylogenetic analysis based on different regions of GDHZ109/2020 (a) and GDYS162/2022 (b). The likely recombination region is shaded in a different color and colored broken lines represent different lineages: green indicates NADC30-like PRRSV (Lineage 1.8), yellow indicates NADC34-like PRRSV (Lineage 1.5), and blue indicates JXA1-like PRRSV (Lineage 8.7). (c and d) Maximum likelihood phylogenetic trees inferred for the different recombinant regions. The ML tree was reconstructed using the best-fit nucleotide substitution model system. 1,000 bootstraps were evaluated to assess support values. NADC30-like PRRSV (Lineage 1.8) is indicated in green, NADC34-like PRRSV (Lineage 1.5) in yellow, and JXA1-like PRRSV (Lineage 8.7) in blue. The red dots indicate strains sequenced in this study.

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