An Inactivated Novel Trivalent Vaccine Provides Complete Protection against FAdV-4 Causing Hepatitis-Hydropericardium Syndrome and FAdV-8b/-11 Causing Inclusion Body Hepatitis

Figure 6

Representative histopathological lesions in tissues from chickens in each group after challenging with FAdV-4, FAdV-8b, and FAdV-11. (a) Histopathological lesions including widened myocardial cell gap and myocardial fiber rupture; severe degeneration and necrosis of hepatocytes; degeneration and necrosis of renal tubular epithelium; mucosal epithelial cell nuclear fragmentation and necrosis in duodenums; and severe depletion and necrosis of lymphocytes in the bursa of Fabricius were observed in the chickens from the unvaccinated and FAdV-4 challenge group. (b) Compared with the massive severe degeneration and necrosis of hepatocytes caused by FAdV-4, the primary histopathological lesions presented by the unvaccinated and FAdV-8b or FAdV-11 challenge groups included multifocal degeneration and necrosis of hepatocytes and mononuclear cell infiltration. However, chickens from the inactivated rFAdV4-fiber/8b + 11 vaccinated group did not present histopathological lesions of HHS and IBH mentioned above (HE staining, original magnification ×400).

(a)

(b)