Research Article

Description of an Equine Hepacivirus Cluster in a Horse Stable in Italy

Table 1

Table of variants in persisted infected horse #142 by comparative full-length genome sequence analysis between ITA/2021/142m and ITA/2021/142o strains. The aa were compared based on their R-group properties. “−” indicates no difference in the aa properties and “+” indicates that the aa belonged in different R-groups.

Genomic position (aa)ORFNucleotide changeAmino acid changeaa properties

434E2TCC⟶CCCSer ⟶ Pro+
437E2CCG⟶CTGPro ⟶ Lau
440E2AAA⟶AGALys ⟶ Arg
444E2GAC⟶AACAsp ⟶ Asn+
470E2AGG⟶AAGArg ⟶ Lys
477E2GCG⟶GTGAla ⟶ Val
561E2TGC⟶TGGCys ⟶ Trp+
1309NS3GGT⟶GTGGly ⟶ Val
1310NS3ATT⟶GTTIle ⟶ Val
2235NS5AAGC⟶AACSer ⟶ Asn+
2261NS5ACGG⟶CAGArg ⟶ Gln+

In bold the nucleotide substitutions. E2 envelope glycoprotein 2; NS3 nonstructural protein 3; NS5A nonstructural protein 5A.