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| Purpose | Type of pigs (sample size) | Source |
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| 1. Estimate model parameters for interconverting neutralization titers and S/P ratios | All types of pigs with neutralization titer ≤ 10 log2 (n = 1,160 for parameter estimation and n = 1,181 for model validation) | S/P ratios and neutralization titers of pigs on seven farms sampled between May 2020 and December 2021 |
| 2. Predict neutralization titers of 1-day-old piglets from neutralization titers of their sows by resampling | Sows (n = 9) and their litters (n = 63) | Neutralization titers of pigs in two farms sampled between May 2020 and September 2021 |
| 3. Predict decline in neutralization titers of MDAs among piglets | Piglets before vaccination (n = 387) | Neutralization titers of pigs on seven farms sampled between May 2020 and December 2021 |
| 4. Sample the S/P ratio distribution of G1 vaccinated sows | G1 sows (n = 168) | S/P ratios of G1 sows in Gifu Prefecture sampled between April and July 2020 |
| 5. Predict changes in VIA levels among fattening pigs | G2 fattening pigs with low, medium, high, and very-high MDA levels at the age of first vaccination (n = 26, 29, 24, and 11, respectively) | S/P ratios of G2 fattening pigs in Aichi and Gifu Prefectures sampled between June and October 2020 |
| 6. Calculate S/P ratio cutoff values for log2 neutralization titers | All types of pigs from farms (n = 1,021) | S/P ratios and neutralization titers of pigs in Gifu Prefecture sampled between April 2020 and March 2021 |
| 7. Validate the prediction of antibody titers among fattening pigs | Fattening pigs at 6 and 26 weeks in a farm 2 years after the start of vaccination (n = 30) | S/P ratios of fattening pigs vaccinated at 6 weeks on a farm in Gifu Prefecture sampled between May and October 2021 |
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