Transboundary and Emerging Diseases

Research Article

Molecular Detection and Distribution of Giardia duodenalis and Cryptosporidium spp. Infections in Wild and Domestic Animals in Portugal

Table 4

Frequency and molecular diversity of Giardia duodenalis and Cryptosporidium spp. in wild and domestic animal species investigated in the present study.


Species	Host	Study area	Genotype	Isolates (n)	Locus	Reference sequence	Stretch	Single nucleotide polymorphisms (SNPs)	GenBank ID

G. duodenalis	Roe deer	MNP	A^a	1	ssu-rRNA	M54878	1–289	A90C, A185R	OQ818646
	Dog	CPW	D	1	ssu-rRNA^b	AF199449	1–248	None	OQ818647
	Iberian wolf	CPW	D	2	ssu-rRNA^b	AF199449	1–293	None	OQ818648
	Iberian wolf	CPW	D	1	ssu-rRNA^b	AF199449	16–284	C262Y	OQ818649
	Iberian wolf	CPW	C	2	gdh	U60984	76–491	C207T	OQ818103
	Iberian wolf	MNP	D	2	gdh	U60986	64–491	T240Y, C375Y, T429Y, G441R, T459W	OQ818104
	Iberian wolf	MNP	D	1	gdh	U60986	81–491	T429C, G441A	OQ818105
	Iberian wolf	CPW, MNP	D	2	bg	AY545647	97–604	A159R, A201G, C207Y	OQ818106
	Red fox	CPW	D	1	ssu-rRNA^b	AF199449	10–293	None	OQ818650
	Red fox	CPW	C	1	gdh	U60984	76–491	None	OQ818107
	Red fox	CPW	C	1	bg	AY545646	4–499	C217T	OQ818108
	Cattle	CPW	E	1	ssu-rRNA^b	AF199448	10–289	None	OQ818651
	Horse	FBR	E	1	ssu-rRNA^b	AF199448	1–289	None	OQ818652
	Sheep	MNP, CPW	E	2	ssu-rRNA^b	AF199448	1–289	None	OQ818653
	Wild boar	MNP	E	1	ssu-rRNA^b	AF199448	1–289	None	OQ818654
C. canis	Iberian wolf	CPW, MNP	Unknown	3	ssu-rRNA	AF112576	525–1030	None	OQ818655
	Red fox	CPE, LM, MNP	Unknown	4	ssu-rRNA	AF112576	525–1030	686InsTG, T739A	OQ818656
C. felis	Iberian lynx	GV	Unknown	1	ssu-rRNA	AF108862	550–1029	C579T	OQ818657
C. ryanae	Red deer	MNP	Unknown	1	ssu-rRNA	MT835148	328–806	None	OQ818658
C. scrofarum	Wild boar	CPE, MNP	–	7	ssu-rRNA	KF597534	285–734	None	OQ818659
	Wild boar	MNP	–	1	ssu-rRNA	KF597534	328–726	438InsTT	OQ818660
C. occultus	Red deer	LM	–	1	ssu-rRNA	MG699179	323–824	484InsAT	OQ818661
C. ubiquitum	Red deer	MNP	Unknown	1	ssu-rRNA	KY052177	21–502	None	OQ818662
	Wild boar	MNP	Unknown	1	ssu-rRNA	KY052177	21–496	None	OQ818663

Note: bg, β-giardin; gdh, glutamate dehydrogenase; Ins, insertion; R, A/G; ssu-rRNA, small subunit ribosomal RNA; W, A/T; Y, C/T. ^aCharacterized as subassemblage AI. ^bTaxonomy and reference sequences as proposed by Plutzer et al. [205]. Mentioned study areas: MNP, Montesinho Natural Park; CPW, Central Portugal West; CPE, Central Portugal East; GV, Guadiana Valley; LM, Lousã Mountains; FBR, Faia Brava Reserve.