Development and Evaluation of the Biological Adjuvant Bivalent Vaccine for Preventing Newcastle Disease and Infectious Bursal Disease

Figure 11

The mRNA expression level of inflammatory cytokines in white cells. Inflammatory cytokines IL-1β, IL-4, IL-17, IFN-α, IFN-β, and IFN-γ were analyzed in the white cells by real-time PCR at 7, 14, 21, and 28 days after immunization. Lane 1: rClone30-VP2L-chGM-CSF; Lane 2: rClone30-VP2L; Lane 3: IBDV-B87; Lane 4: rClone30-chGM-CSF; Lane 5: rClone30; Lane 6: PBS. Data were analyzed and expressed as mean ± SD, ^#P < 0.05, ^##P < 0.01 vs rClone30-VP2L-treated chickens and expressed as mean ± SD, ^∗∗P < 0.01 vs rClone30-treated chickens.