Development and Evaluation of the Biological Adjuvant Bivalent Vaccine for Preventing Newcastle Disease and Infectious Bursal Disease

Table 1

Primers used in the amplification of the target genes.

Primers	Primer sequences (5′-3′)
P1	CCG​CGGGGG​CCA​CCA​TGA​CAA​ACC​TGC​AAG​ATC
P2	CCG​CGGTTC​TAC​CCG​TAT​TTT​TTC​TTA​ATT​AGG​TGT​ACT​CCC​TTG​TT
P3	CCG​CGGGCC​ACC​ATG​ACA​AAC​CTG​CAA​GAT​C
P4	GTT​TAA​ACCTT​CTA​CCC​GTA​TTT​TTT​CTT​AAT​TAG​GTG​TAC​TCC​CTT​GTT

Note. Italic parts are the recognition sites of restriction endonuclease SacII and PmeI. P1–P4 are used for cloning the VP2 neutralizing epitope antigen (VP2L) gene.