Development and Evaluation of the Biological Adjuvant Bivalent Vaccine for Preventing Newcastle Disease and Infectious Bursal Disease

Table 2

Experimental design for chicken immunization.

Groups of vaccine	Administration	Dose (μl)	Number of chickens	Age (days) at vaccination		Days of challenged
				1st	2nd
rClone30-VP2L-chGM-CSF	Intramuscularly	200	10	14	28	42
rClone30-VP2L	Intramuscularly	200	10	14	28	42
IBDV-B87 vaccine	Oculonasal route	200	10	14	28	42
rClone30-chGM-CSF	Intramuscularly	200	10	14	28	42
rClone30	Intramuscularly	200	10	14	28	42
PBS (negative control)	Intramuscularly	200	10	14	28
PBS (challenge control)	Intramuscularly	200	10	14	28	42