Isolation, Identification, Sequence Analysis, and Pathogenicity of a CIAV Strain from Aegypius monachus

Table 1

Sequences of primers used in this study.

No.	Primers	The sequences of the primers (5′ ⟶ 3′)	Sizes (bp)
1	CIAV-F	AGA​AAG​TCA​AGA​TGG​ACG​AAT​C	440
	CIAV-R	ATC​TTC​TTG​GAG​AGC​GTT​CAT
2	ALVA-F	CGG​AGA​AGA​CAC​CCT​TGC​T	715
	ALVA-R	GCA​TTG​CCA​CAG​CGG​TAC​TG
3	ALVB-F	CGG​AGA​AGA​CAC​CCT​TGC​T	515
	ALVB-R	GTA​GAC​ACC​AGC​CGG​ACT​ATC
4	ALVJ-F	CGG​AGA​AGA​CAC​CCT​TGC​T	422
	ALVJ-R	CGA​ACC​AAA​GGT​AAC​ACA​CG
5	REV-F	CAT​ACT​GGA​GCC​AAT​GGT​T	320
	REV-R	AAT​GTT​GTA​GCG​AAG​TAC​T
6	MDV-F	GCC​TTT​TAT​ACA​CAA​GAG​CCG​AG	560
	MDV-R	TTT​ATC​GCG​GTT​GTG​GGT​CAT​G
7	CIAV-com-F1	GCA​TTC​CGA​GTG​GTT​ACT​ATT​CC	842
	CIAV-com-R1	CGT​CTT​GCC​ATC​TTA​CAG​TCT​TAT
8	CIAV-com-F2	CGA​GTA​CAG​GGT​AAG​CGA​GCT​AAA	990
	CIAV-com-R2	TGC​TAT​TCA​TGC​AGC​GGA​CTT
9	CIAV-com-F3	ACG​AGC​AAC​AGT​ACC​CTG​CTA​T	802
	CIAV-com-R3	CTG​TAC​ATG​CTC​CAC​TCG​TT

Note. Primers 1–6 were used to detect viral DNA in clinical samples; primers 7–9 were used to amplify the full-length of CIAV genome.