Molecular Detection and Genetic Characterization of Bovine Kobuvirus (BKV) in Diarrhoeic Calves in a Central Italy Herd

Table 1

Primer pair candidates and probes used for detecting different viruses using real-time PCR.

Forward primer	5′-CAAATCTCGYCGCTCATTGTTC-3′	Amplicon of 114 bp	BKV
Reverse primer	5′-GTT​GAG​TAG​AAG​TAA​TCT​GGG​TTC​T-3′
Probe	5′-JUN-TCCCTACATCCGAACTCCAGGCT-QSY-3′
Forward primer	5′-CTG​GAA​GTT​GGT​GGA​GTT-3′	Amplicon of 85 bp	BCoV
Reverse primer	5′-ATT​ATC​GGC​CTA​ACA​TAC​ATC-3′
Probe	5′-FAM-CCTTCATATCTATACACATCAAGTTGTT-BHQ-3′
Forward primer	5′-GRA-GTC-GTC-ART-GGT-TCG-AG-3′	Amplicon of 208 bp	BVDV
Reverse primer	5′-TCA-ACT-CCA-TGT-GCC-ATG-TAC-3′
Probe	5′-FAM-TGC-YAY-GTG-GAC-GAG-GGC-ATG-C-TAMRA-3′