Detection of <i>Clostridium perfringens</i> Using Novel Methods Based on Recombinase-Aided Amplification Assay-Assisted CRISPR/Cas12a System

<div>Feasibility verification of the RAA-CRISPR/Cas12a-based <i>C. perfringens</i> detection methods. (a) Efficiency evaluation of designed four RAA primer sets using gel electrophoresis. M, 500 DNA marker; lanes 1–4, RAA product of primer set 1, 2, 3, and 4, respectively. (b) Target sequences and their corresponding primer sets of six crRNAs were used in this study. (c) Screening the optimal combination of primer set and crRNA using RAA-CRISPR/Cas12a-FL assay through a multifunctional microplate reader (upper) or a UV flashlight (below). (d) Verifying the feasibility of the RAA-CRISPR/Cas12a-LFS method using the F1/R1-CR3 combination. 1, RAA template is H<sub>2</sub>O; 2, RAA template is <i>C. perfringens</i> genomic DNA.</div>

Transboundary and Emerging Diseases

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Figure 2: Detection of <i>Clostridium perfringens</i> Using Novel Methods Based on Recombinase-Aided Amplification Assay-Assisted CRISPR/Cas12a System 

Transboundary and Emerging Diseases

Detection of Clostridium perfringens Using Novel Methods Based on Recombinase-Aided Amplification Assay-Assisted CRISPR/Cas12a System

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