Research Article
Detection of Clostridium perfringens Using Novel Methods Based on Recombinase-Aided Amplification Assay-Assisted CRISPR/Cas12a System
Figure 3
Optimization of the assay time of RAA-CRISPR/Cas12a system in the detection of C. perfringens. The RAA-CRISPR/Cas12a-FL assays were conducted using 1 × 105 copies/μL of C. perfringens genomic DNA as the template to obtain the optimal time of RAA reaction (a) and Cas12a cleavage (b), and the fluorescence signals were detected with a multifunctional microplate reader (upper) or a UV flashlight (below). n = 3 technical replicates; bars represent mean ± SEM.
| (a) |
| (b) |