Detection of Clostridium perfringens Using Novel Methods Based on Recombinase-Aided Amplification Assay-Assisted CRISPR/Cas12a System

Figure 5

Evaluation of the specificity of RAA-CRISPR/Cas12a-based methods for detecting C. perfringens. Six C. perfringens strains and 10 other related zoonotic bacterial pathogens were used to evaluate the specificity of RAA-CRISPR/Cas12a-FL and RAA-CRISPR/Cas12a-LFS methods in the detection of C. perfringens, and the fluorescence signals were detected with a multifunctional microplate reader (upper) or a UV flashlight (below). 1, Salmonella typhimurium; 2, Vibrio parahaemolyticus; 3, Edwardsiella piscicida; 4, Escherichia coli; 5, Staphylococcus aureus; 6, Pseudomonas aeruginosa; 7, Bacillus cereus; 8, Aeromonas hydrophila; 9, Vibrio vulnificus; 10, Vibrio harvey; 11, C. perfringens strain MLa; 12, C. perfringens strain MLb; 13, C. perfringens Type A; 14, C. perfringens Type B; 15, C. perfringens Type C; 16, C. perfringens Type D. n = 3 technical replicates; bars represent mean ± SEM.

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