The Genetic Diversity and Interspecific Transmission of Circovirus in Rhizomys sinensis in Guangdong, Southern China

Figure 3

Identification of bamboo rat circoviruses in Rhizomys sinensis. (a) Genome schematic illustrating bamboo rat circoviruses’ major open reading frames (ORFs) characteristics. Members of the species have two major ORFs and a conserved nonnucleotide motif marking the origin of replication. (b) Nucleotide sequence alignment of the partial region from all bamboo rat circoviruses was the only region in which single base deletions were present. (c and d) Phylogenetic analysis of the identified bamboo rat circoviruses by MEGA 11 software and the nucleotide sequence features observed by CLC sequence viewer software on the right side. The trees were constructed from the nucleotide sequences of the complete genome (c) and ORF2 (d). Bootstrap values expressed as percentages of 1,000 replications are shown at the branch nodes. The red circles represent bamboo rat circoviruses in this study. Nucleotide sequence features corresponding to each clade are displayed to the right of the phylogenetic tree, with regions marked by red boxes.

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