Research Article
A Single Mutation of VP2 is Responsible for the Lethality and Antigenicity Differences between Novel Variant and Very Virulent IBDV Strains
| | Primers | Sequence (5′-3′) | Purpose |
| | pCA-EcoR1-F | TCTCATCATTTTGGCAAAGAATTC | Point mutation | | pCA-Bgl2-R | ATTTTTGGCAGAGGGAAAAAGATCT | | | HLJVP2-D279N-F | CACCAGAGCTGTGGCCGCAAACAATGGGCTAACGGCCGGCAC | | | HLJVP2-D279N-R | GTGCCGGCCGTTAGCCCATTGTTTGCGGCCACAGCTCTGGTG | | | SHG19VP2-N279D-F | AGAGCTGTAGCTGCAGACAATGGGCTGACGG | | | SHG19VP2-N279D-R | CCGTCAGCCCATTGTCTGCAGCTACAGCTCT | | | PCA-HLJVP2-F | TCTCATCATTTTGGCAAAGAATTCGCCACCATGACAAACCTGCAAGATC | | | PCA-HLJVP2-R | TTGGCAGAGGGAAAAAGATCTTTAAGCGTAATCTGGAACATCGTATGGGTATGCTCCTGCAATCTTCAG | | | IBDV-Probe | FAM-CGGCGTCCATTCCGGACGAC-BHQ-1 | RT-qPCR for IBDV | | VP5-F | GAGCCTTCTGATGCCAACAAC | | | VP5-R | CAAATTGTAGGTCGAGGTCTCTGA | | | 28S-Probe | FAM-AGGACCGCTACGGACCTCCACCA-TAMRA | RT-qPCR for 28s rRNA | | 28S-F | GGCGAAGCCAGAGGAAACT | | | 28S-R | GACGACCGATTTGCACGTC | | | IL-1β-F | CCGAGGAGCAGGGACTTT | RT-qPCR for IL-1β | | IL-1β-R | AGGACTGTGAGCGGGTGTAG | | | β-actin-F | TCCACCGCAAATGCTTCTAAAC | RT-qPCR for β-actin | | β-actin-R | CTGCTGACACCTTCACCATTCC | |
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