ELISA Methods Based on Monoclonal Antibodies for the Serological Diagnosis of Lumpy Skin Disease
Table 1
Goat sera collected at different time points after experimental infection with GTPV, strain “V/103.”
Animal ID
Infection route
dpi
VNT (titer)
Capripox DA ELISA (ID.vet) (S/P%)
MAbs-based competitive ELISA PI values
MAbs-based indirect ELISA OD values
Z/253
Intravenous and subcutaneous
0
<1 : 10
−1
0
0
7
<1 : 10
−1
4
0.035
10
1 :
−1
45
0.105
Z/254
Intravenous and subcutaneous
7
1 :
0
37
0.004
10
1 :
16
0.034
Z/255
Intravenous and subcutaneous
0
<1 : 10
0
0
0
7
<1 : 10
0
0
0
10
1 :
0.153
Z/256
Intranasal
0
<1 : 10
−1
0
0
7
<1 : 10
−1
0
0.045
15
1 :
23
Z/257
Intranasal
0
<1 : 10
−1
0
0
7
<1 : 10
−1
0
0.008
28
1 :
Z/258
Intranasal
0
<1 : 10
−1
0
0.132
7
<1 : 10
−1
0
0.106
15
1 :
1
28
1 :
Z/259
In contact
0
<1 : 10
6
0
0.018
7
<1 : 10
1
0
0.01
15
<1 : 10
−1
0
0.006
21
<1 : 10
−1
34
0.023
23
1 :
0
0.109
Z/260
In contact
0
<1 : 10
−1
0
0
7
<1 : 10
−1
0
0.005
15
<1 : 10
−1
0
0.003
21
<1 : 10
0
0
0
28
1 :
15
0.145
The infection route and the results of the virus neutralization test (VNT), the ID.vet Capripox DA ELISA test (S/P%), and the new competitive and indirect ELISAs based on monoclonal antibodies are reported. The positive results are highlighted with the symbol, according to the cutoff value established for each test.
