ELISA Methods Based on Monoclonal Antibodies for the Serological Diagnosis of Lumpy Skin Disease
Table 2
Sheep sera collected at different time points after experimental infection with two strains of SPPV.
Challenge virus
Animal ID
Infection route
dpi
VNT (titer)
Capripox DA ELISA (ID.vet) (S/P%)
MAbs-based competitive ELISA (PI values)
MAbs-based Indirect ELISA (OD values)
SPPV-Egypt/2018
S-01
Intravenous
0
<1 : 10
0
0
0
7
<1 : 10
15
0
14
1 :
0.185
21
1 :
28
1 :
SPPV-Egypt/2018
S-02
Intravenous
0
<1 : 10
0
0
0.018
7
<1 : 10
45
0.012
14
1 : 13
22
SPPV-Egypt/2018
S-03
Intravenous
0
<1 : 10
1
0
0
7
<1 : 10
9
0,03
14
1 :
23
21
1 :
28
1 :
SPPV-Egypt/2018
S-04
Intranasal
0
<1 : 10
0
0
0.022
7
<1 : 10
0
0
0
14
<1 : 10
5
SPPV-Egypt/2018
S-05
Intranasal
0
<1 : 10
0
0
0.099
7
<1 : 10
1
2
0.088
14
1 : 13
25
SPPV-Egypt/2018
S-06
Intranasal
0
<1 : 10
0
0
0
7
<1 : 10
2
29
0
14
1 :
12
SPPV-India/2013
S-09
Intravenous
0
<1 : 10
0
0
0.009
7
<1 : 10
21
37
0
14
1 : 13
16
SPPV-India/2013
S-12
Intranasal
0
<1 : 10
1
0
0
7
<1 : 10
0
0
0
14
<1 : 10
SPPV-India/2013
S-14
Intranasal
0
<1 : 10
−1
0
0
7
<1 : 10
−1
3
0
14
<1 : 10
10
21
1 :
28
1 :
The virus used for the infection and the infection route are reported, together with results of the virus neutralization test (VNT), the ID.vet Capripox DA ELISA test (S/P%), and the new competitive and indirect ELISAs based on monoclonal antibodies. The positive results are highlighted with the symbol according to the cutoff value established for each test.
