ELISA Methods Based on Monoclonal Antibodies for the Serological Diagnosis of Lumpy Skin Disease

Table 3

Isotype and reactivity profile of the 14 MAbs reactive in western blotting against a 35 kDa viral protein.

Monoclonal antibody	Indirect ELISA	MAbs reciprocal competition
	Neethling LSDV (OD value)	PI vs. 3H5-PO	PI vs. 2C6-PO	PI vs 2F10-PO	Isotype
2C6	2.0				IgG1
3H5	1.9			54^+	IgG1
5C8	1.5	60^+	64^+	53^+	IgG2a
2F10	2.0	37	40		IgG1
7B7	1.8	31	31		IgG1
4E8	1.6	26	36		IgG2a
6G4	2.1	28	35		IgG1
2A9	2.1	24	14		IgG1
2A6	2.2	22	17		IgG1
2C9	2.1	21	17	66^+	IgG1
2F12	2.0	20	19	58^+	IgG1
8C4	1.8	13	27	52^+	IgG1
3D12	2.1	1	11	34	IgG1
2E11	1.9	12	12	30	IgG1

The table presents the OD values of indirect ELISA obtained by each MAb against the Neethling strain, and the percentage of inhibition (PI) of competitive ELISA between the tested MAbs (competitor) and three peroxidase- (PO-) conjugated MAbs. High-competition value is shown with symbol, partial competition is indicated with + symbol, and the numbers with no symbol show low or no competition.