A Combined Method Based on the FIPV N Monoclonal Antibody Immunofluorescence Assay and RT-nPCR Method for the Rapid Diagnosis of FIP-Suspected Ascites

Table 1

The results of ascites samples by IFA and RT-nPCR.

Sample names	IFA	RT-nPCR FCoV 3′UTR gene	RT-nPCR FCoV S gene	RT-nPCR FCoV N gene
1	+	+	+	+
2	+	+	+	+
3	+	+	+	+
4	+	+	+	−
5	+	+	−	+
6	+	+	+	+
7	+	+	−	+
8	+	−	+	+
9	+	+	+	+
10	+	+	+	+
11	−	−	−	−
12	+	−	+	+
13	+	+	−	+
14	+	+	−	−
15	+	+	+	+
16	+	+	+	−
17	+	+	−	+
18	+	+	+	−
19	+	+	−	+
20	+	−	−	−
21	+	+	+	+
22	+	+	−	−
23	+	+	−	+
24	+	+	+	+
25	+	+	−	−
26	+	+	+	+
27	+	+	+	+
28	+	+	+	+
29	+	+	+	+
30	−	+	+	+
31	+	+	−	+
32	+	+	+	+
33	+	+	+	+
34	+	+	+	+
35	+	+	+	+
36	−	−	−	−

Note. “+”: represents positive; “−”: represents negative.