Identification of a Novel Astrovirus Associated with Bovine Respiratory Disease

Figure 2

Detection of the novel bovine astrovirus nucleic acid in lungs from cattle with respiratory disease by in situ hybridization (ISH). Signal for the novel BAstV ranged from patches in 21-24401, indicated by arrows (a), to intensely diffuse in 20-25551 (b), 100x. Pinpoint signals from 21-24401 were detected in the cytoplasm of cells that corresponded to large alveolar macrophages, as well as mononuclear cells indicated by arrows (c), 400x. Diffuse, intense novel BAstV ISH signals obscured entire cells in sample 20-25551 indicated by arrows (d), 400x. No signal was detected in the bronchial epithelium, indicated by arrow, (e) or neutrophils, indicated by arrow, (f), in 20-25551 and 21-24401, respectively 400x. A negative control is inserted in (e).

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