Evidence of Antibodies against the West Nile Virus and the Usutu Virus in Dogs and Horses from the Southeast of France

Table 2

Concentration of anti-IgG antibodies by serum neutralisation testing among ELISA-positive animals. The value of the optical density of the ELISA. The infectious status and animal origins are provided.

Animal species	Department	Location	Code	ELISA flaviviruses POS D0 (%)	SNT WNV titer	SNT USUV titer	Interpretation
Horse	Hérault	Vic-la-Gardiole	EYG30	3.5	NEG	NEG
			EYG44	3.3	NEG	NEG
			EYG46	8.4	1/40	NEG	WNV
		Villeneuve-lès-Maguelone	EYG5	5.8	1/40	1/20	WNV and/or USUV positive
		Fabrègues	EYG60	3.9	NEG	NEG
			EYG98	3.4	NEG	NEG
Dog	Hérault	Mireval	CNH15	4.1	NEG	NEG
			CNH16	3.4	NEG	NEG
			CNH18	3.4	NEG	NEG
			CNH19	3.6	NEG	NEG
			CNH20	3.8	NEG	NEG
			CVG2	3.4	NEG	NEG
			CNH115	3.2	NEG	NEG
			CNH116	4.6	NEG	NEG
			CNH121	3.5	NEG	NEG
			CNH129	11.2	1/80	NEG	WNV
			CNH132	5.7	1/80	1/20	WNV
			CNH135	12.2	1/40	NEG	WNV
			CNH142	3.1	NEG	NEG
			CNH146	3.4	NEG	NEG
		Vic-la-Gardiole	CNH5	3.4	NEG	NEG
			CNH6	3.9	NEG	NEG
			CNH7	3.4	NEG	NEG
			CNH39	3.4	NEG	NEG
			CNH45	3.8	NEG	NEG
			CNH46	3.0	NEG	NEG
			CNH50	4.2	NEG	NEG
			CNH53	3.4	NEG	NEG
		Villeneuve-lès-Maguelone	CNH56	3.1	NEG	NEG
			CNH59	4.0	NEG	NEG
			CNH61	4.1	NEG	NEG
			CNH64	3.6	NEG	NEG
			CNH71	3.6	NEG	NEG
			CNH76	3.5	NEG	NEG
			CNH77	3.2	NEG	NEG
			CNH84	4.0	NEG	NEG
			CNH85	3.3	NEG	NEG
			CNH102	3.7	NEG	1/80	USUV
	Bouches-du-Rhône	Le Paty	SD10	7.7	1/40	NEG	WNV
			SD14	4.9	NEG	1/40	USUV
			SD15	12.6	1/40	NEG	WNV
			SD18	3.8	NEG	1/160	USUV
			SD24	4.6	NEG	1/40	USUV
			SD26	4.8	1/40	1/20	WNV and/or USUV positive
			SD31	12.1	1/80	1/20	WNV
			SD39	8.0	1/40	NEG	WNV
			I8	6.6	1/80	1/160	WNV and/or USUV positive
		Miramas	GA04	7.9	1/80	NEG	WNV
			GA26	6.8	1/40	NEG	WNV
		Salon de Provence	MM4	3.3	1/40	1/40	WNV and/or USUV positive
			MM5	3.5	1/20	NEG	WNV
			MM11	7.0	1/40	1/20	WNV and/or USUV positive
			MM12	5.2	1/40	1/40	WNV and/or USUV positive
	Var	Hyères	GF3	6.5	1/40	NEG	WNV
			GF9	7.5	1/160	1/80	WNV and/or USUV positive
			GF15	3.1	NEG	NEG
			GF16	3.9	1/80	1/20	WNV
			GF18	3.3	1/40	1/20	WNV and/or USUV positive
			GF19	4.5	1/80	NEG	WNV
			GF20	4.9	1/80	1/20	WNV

Doubtful interpretation: animals were positive for either WNV and/or USUV.