Isolation and Identification of a Genotype C Bovine Parainfluenza Virus Type 3 and Its Pathogenicity in Albino Guinea Pigs

Figure 2

Isolation and identification of the BPIV3 strain BPIV3-SX-2021. (a) Cytopathic effects (CPE) of the BPIV3-SX-2021 isolates in infected MDBK cells, such as shrinkage, rounding, and nonadherence, were observed in MDBK cells infected with BPIV3-SX-2021 at 48 h.p.i. No CPE was observed in uninfected cells. (b) RT-PCR analyses of BPIV3-SX-2021 at F1, F2, F3, F4, and F5 using the BPIV-F/R. (c) Indirect immunofluorescence assay of BPIV3-SX-2021-infected MDBK cells using the anti-NP antibody. (d) The viral protein NP was detected with western blotting using an anti-NP Polyclonal antibody. (e) Plaque morphology of the BPIV3-SX-2021 in MDBK cells at 96 h.p.i. (f) The growth curves for the viruses and viral titers were determined with a TCID₅₀ assay. The data represent the mean ± SD for three independent experiments.

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