Gossypol Affects Viral Replication by Inhibiting Pseudorabies Virus Adsorption

Figure 1

The anti-PRV effect of gossypol. (a, b) The cell viability of NIH-3T3 (a) and PK-15 (b) cells treated with gossypol (0–10 µM) for 12, 24, 36, or 48 hr, as detected by CCK-8. and . (c) PK-15 cells were pretreated with the indicated concentrations of gossypol for 4 hr and then inoculated with gossypol-containing rPRV HN1201-EGFP-Luc (MOI = 0.01) virus solution at 37°C for 1 hr. The infecting inoculum was replaced with gossypol-containing complete medium for 24 hr, and the cell fluorescence was detected under a fluorescence microscope. Scale bar, 200 µm. (d) EGFP-positive PK-15 cells were detected by flow cytometry (cells were treated as described above in part (c)). and . (e) Western blot analysis of PK-15 cells that were treated described above as in part (c) but infected with PRV HN1201 (MOI = 1), conducted with the indicated antibodies.

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