Gossypol Affects Viral Replication by Inhibiting Pseudorabies Virus Adsorption

Figure 2

Anti-PRV activity of gossypol was independent of mtDNA leakage and IFN-β production. (a, b) Confocal laser microscopy observation of the mitochondrial morphology (a) and a statistical analysis of the mitochondrial length at specific time points (0, 2, 6, 12, 24, and 36 hr), conducted with ImageJ software, (b) of PK-15 cells after their treatment with 3 µM gossypol. Scale bar, 10 µm; . (c, d) PK-15 cells were treated with the indicated concentrations of gossypol for 24 hr, and then stained with JC-1. The change in mitochondrial membrane potential was observed by confocal laser microscopy (c), and the ratio of red and green fluorescence intensity of the images was analyzed using ImageJ software (d). Scale bar, 20 µm; . (e, f) qPCR Assay of intracellular mtDNA (e) and IFN-β (f) expression change in PK-15 cells that were treated with the indicated concentrations of gossypol (0, 0.1, 0.3, 1, or 3 µM) for 24 hr. NS, not significant, .

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