Gossypol Affects Viral Replication by Inhibiting Pseudorabies Virus Adsorption

Figure 3

Gossypol did not exert anti-PRV effects by stimulating ROS. (a) The level of ROS in PK-15 cells that were treated with the indicated concentrations of gossypol for 24 hr was detected with a Reactive Oxygen Species Assay kit; the cells were observed and photographed under a confocal laser microscope. Scale bar, 20 µm. (b) PK-15 cells were treated with DMSO, gossypol (3 µM), gossypol (3 µM) +NAC (0.1 mg/mL), or gossypol (3 µM) +GSH (1 mg/mL) and then inoculated with rPRV HN1201-EGFP-Luc (MOI = 0.01). The viral proliferation after 24 hr was observed under a fluorescence microscope. Scale bar, 100 µm. (c) Cells were treated and inoculated as described above in part (b), and the proportion of EGFP-positive cells was determined by flow cytometry .

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