Gossypol Affects Viral Replication by Inhibiting Pseudorabies Virus Adsorption

Figure 4

Anti-PRV effect of gossypol was ATP-independent. (a, b) ATP detection after the treatment of PK-15 (a) and NIH-3T3 (b) cells with the indicated concentrations of gossypol (0, 0.1, 0.3, 1, or 3 µM) for 24 hr. and . (c, d) ATP detection at different lengths of time (0, 6, 12, or 24 hr) after the treatment of PK-15 (c) and NIH-3T3 (d) cells with 3 µM gossypol. and . (e) PK-15 cells were treated with the indicated concentrations of ATP and inoculated with rPRV HN1201-EGFP-Luc (MOI = 0.1). The viral proliferation after 24 hr was observed under a fluorescence microscope. Scale bar, 200 µm. (f) Cells were treated and inoculated as described above in part (e), and the proportion of EGFP-positive cells after 24 hr was determined by flow cytometry. , . (g) PK-15 cells were treated with DMSO, gossypol (3 µM), or gossypol (3 µM) +ATP (100 µM) and then inoculated with rPRV HN1201-EGFP-Luc (MOI = 0.01). The cells were observed and photographed 24 hr later under a fluorescence microscope. Scale bar, 100 µm. (h) Cells were treated and inoculated as described above in part (g), and the proportion of EGFP-positive cells was determined by flow cytometry. .

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