Transboundary and Emerging Diseases / 2023 / Article / Tab 1 / Research Article
Flying Syringes for Emerging Enzootic Virus Screening: Proof of Concept for the Development of Noninvasive Xenosurveillance Tools Based on Tsetse Flies Table 1 Characteristics of the six viruses selected for tsetse fly oral infection.
Virus name (abbreviation) Family/Genus Presence of envelope Genome features Strain Virus titer reported in vertebrate hosts Final virus titer used for blood meals Chikungunya (CHIKV) Alphaviridae /Alphavirus Yes ssRNA(+) LR2006_OPY1 [26 ] 101 –108 PFU/mL [27 ] 106 PFU/mL Dengue 2 (DENV-2) Flaviviridae /Flavivirus Yes ssRNA(+) 16681 [28 ] 102 –107 PFU/mL [29 ] 106 FFU/mL Zika (ZIKV) Flaviviridae /Flavivirus Yes ssRNA(+) PF_25013-18 [30 ] 102 –106 PFU/mL [31 ] 106 PFU/mL African swine fever (ASFV) Asfaviridae /Asfivirus Yes dsDNA LIV13/33 [32 ] 102.5 –103.5 HAD50 /mL in wild animals [33 ]8 HAD50 /mL in domestic animals [34 ] 103 HAD50 /mL Peste des petits ruminants (PPRV) Paramyxoviridae/Morbillivirus Yes ssRNA(−) Maroc2008 [35 ] 106 –109 RNA copies/mL in domestic animals [36 ] 107 RNA copies/mL Bluetongue (BTV-8) Reoviridae/Orbivirus No dsRNA Serotype - 8 Italy [37 ] 106 –108 particles/mL in wild animals [38 ]4 –107 DL50 in domestic animals [39 ] 105.2 TCID50 /mL
ssRNA(+), positive-sense single-stranded RNA; ssRNA(−), negative-sense single-stranded RNA; dsRNA, double-stranded RNA; dsDNA, double-stranded DNA; PFU, plaque forming unit; FFU, focus forming unit; HAD, hemadsorbing dose; TCID, tissue culture infectious dose.
