Import Risk Analysis of the Decapod Iridescent Virus 1 (DIV1) Applied in South Korea: Qualitative Risk Review and Institutional Improvement Plans

Table 5

The criteria of susceptible species and DIV1 susceptible species.

(a) The criteria of susceptible species of DIV1
Classification		Criteria	Definition
Stage 1		(i) Naturally infected species (including outbreaks in aquaculture)	(i) Infections caused by noninvasive and invasive laboratory experiments are not considered
Stage 2		(i) DIV1 had detected by nested-PCR method of Qiu et al. [1] or real-time PCR method of Qiu et al. [7] in WOAH disease card of DIV1	(i) Must be detected by a globally accepted detection method.; (ii) There is no WOAH Aquatic Manual for DIV1 yet
Stage 3		(1) The pathogen is multiplying in the host, or the pathogen is developing in or on the host; (2) A viable pathogen is isolated from a susceptible species presented or transmitted to a pure infective individual; (3) Infection results in clinical or pathological changes; (4) The specific location of the pathogen matches the expected target tissue	(1) When proving that pathogens exist in the host through analysis such as transmission electron microscopy (TEM); (2) In case of transmission (horizontal infection) from a susceptible species that satisfies (1) of Stage 3, or the disease occurred from a completely natural environment (wild); (3) In the case of the reported clinical or pathological lesions from a susceptible species that satisfies (1) of Stage 3 in the reported susceptible species; (4) When the exact target tissue is identified by (1) of Stage 3 and the pathogen is detected from that tissue
(b) DIV1 susceptible species
Classification		General name (scientific name)	Basis	References
Completely susceptible	Natural infection	Redclaw crayfish (Cherax quadricarinatus)	Stages 1–2 and (1) of Stage 3	[2]
		Oriental river prawn (Macrobrachium nipponense)	Stages 1–2 and (2), (3), (4) of Stage 3	[3]
		Giant freshwater prawn (Macrobrachium rosenbergii)	Stages 1–2 and (1) of Stage 3	[3]
		Giant tiger prawn (Penaeus monodon)	Stages 1–2 and (2), (4) of Stage 3	[8, 18]
		(Macrobrachium superbum)^a	Stages 1–2 and (2), (4) of Stage 3	[3]
		Kuruma prawn (Penaeus japonicus)	Stages 1–2 and (2), (4) of Stage 3	[19]
		Fresh prawn (Penaeus chinensis)	Stages 1–2 and (2), (4) of Stage 3	[1, 19]
		Polychaetes	Stages 1–2	[19, 20]
		Cladocera spp.	Stages 1–2	[3, 17]
	Complex infection	Whiteleg shrimp (Penaeus vannamei)	Stages 1–2 and (1) of Stage 3	[1, 7]
		Red swamp crayfish (Procambarus clarkii)	Stages 1–2 and (2), (3), (4) of Stage 3	[2, 3]
		Oriental prawn (Exopalaemon carinicauda)	Stages 1–2 and (2), (4) of Stage 3	[17, 21]
Incompletely susceptible	Experimental infection	Chinese mitten crab (Eriocheir sinensis)	Stage 2 and (4) of Stage 3	[22]
		Striped shore crab (Pachygrapsus crassipes)	Stage 2 and (4) of Stage 3	[22]
		Banana prawn (Penaeus merguiensis)	Stage 2 and (4) of Stage 3	[23]

Vectors. Intramuscular injection. ^aAlthough the number of infected individuals (1 of 1) and copies of the virus (1 × 10–1.18 × 10) are relatively small and lower compared to other susceptible species, the WOAH designation criteria for susceptible species include the number of samples and separate standards for viral copies, etc., so in this study, M. superbum is also considered to be a completely susceptible species. PCR, polymerase chain reaction.