Isolation and Characterization of Porcine Sapelovirus from the PDCoV-Positive Sample and Its Molecular Epidemiology in Henan Province, China

Figure 1

Isolation and identification of PSV HNHB-01 strain. (a–d) The CPE of LLC-PK and ST cells infected with PSV strain HNHB-01. (a) Mock-inoculated with LLC-PK cell culture. (b) PSV- inoculated LLC-PK cells. (c) Mock-inoculated with ST. (d) PSV- inoculated ST cells. (e–h) Detection of PSV isolate HNHB-01 in LLC-PK and ST cells by IF staining using anti-PSV VP1 specific monoclonal antibody. (f) IF staining of PSV HNHB-01-inoculated LLC-PK cells showing large numbers of IF-stained cells. (h) IF staining of PSV HNHB-01-inoculated ST cells showing IF-positive staining. (e and g) IF staining of mock-inoculated LLC-PK and ST cells showing no IF-positive cells. (i) Electron micrographs of PSV HNHB-01-inoculated ST cells. (j and k) Plaques of purified PSV HNHB-01 virions. (l) Virus titers of PSV HNHB-01 on ST cells during serial passage.

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