Research Article

Isolation and Characterization of Porcine Sapelovirus from the PDCoV-Positive Sample and Its Molecular Epidemiology in Henan Province, China

Figure 3

Phylogenetic analysis based on the complete sequences from different PSV strains. The phylogenetic tree was constructed from the aligned nucleotide sequences using the neighbour-joining method with MEGA 6.06 software (http://www.megasoftware.net) based on the whole genomes from PSV HNHB-01 strains (P5, P30, P60, and P100) and the other PSV strains obtained from NCBI. Bootstrap values were calculated with 1,000 replicates. Reference sequences obtained from GenBank are indicated by strain names and GenBank accession numbers. The black triangles indicate the PSV strains that identified by our laboratory. The China PSVs in this study were all in blue boxes.