Dynamics of Serological and Mucosal Antibody Responses against African Swine Fever Viruses in Experimentally Infected Pigs

Figure 2

Serological IgG responses (indicated as black squares with a solid line) and mucosal sIgA responses (indicated as red squares with a dotted line) to ASFV recombinant proteins in the pigs orally and nasally inoculated with 10⁵ HAD₅₀ of the virulent field genotype II isolate HLJ/18. ELISA plates coated with B646L/P72 (a), E183L/P54 (b), CP204L/P30 (c), KP177R/P22 (d), K78R/P10 (e), and EP402R/CD2v (f) proteins were used to determine specific antibody responses at different DPIs. The serological IgG responses were analysed using mixed serum samples from four pigs. Positive conversion was identified when the P/N0 value was ≥2.1 and is indicated by a grey dotted line. The data represent the mean and SD of three parallel experiments.

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