Dynamics of Serological and Mucosal Antibody Responses against African Swine Fever Viruses in Experimentally Infected Pigs

Figure 5

sIgA responses to ASFV recombinant proteins in contact transmission-infected pigs based on oral swab samples. ELISA plates coated with CP204L/P30 (a), E183L/P54 (b), K78R/P10 (c), KP177R/P22 (d), and EP402R/CD2v (e) proteins were used to determine specific antibody responses at various DPIs. Contact 1 and contact 2, contact 3 and contact 4, and contact 5 and contact 6 were cohoused with pigs that were intramuscularly inoculated with 10⁶ TCID₅₀ of SD/DY-I/21, 10³ TCID₅₀ of SD/DY-I/21, and 10⁶ TCID₅₀ of HLJ/HRB1/20 from the first day of challenge, respectively. Positive conversion was identified when the P/N0 value was ≥2.1 and is indicated by a grey dotted line.

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