Rapid Visual Detection of Glaesserella parasuis with a Real-Time Recombinase-Aided Amplification Assay

Figure 2

The position of the real-time RAA probe and primers within the aligned infB sequences from G. parasuis. The information of the sequences is listed on the left. The primer pair and probe were shaded with purple and green, respectively. Nucleotide residues that match the majority are indicated by “”. Nucleotide deletions are indicated by triangle. The dT-fluorophore residue (FAM-dT) and dT-quencher residue (BHQ1-dT) were marked by the green and blue circles, respectively.