Rapid Visual Detection of Glaesserella parasuis with a Real-Time Recombinase-Aided Amplification Assay

<div>The specificity analysis of the G. parasuis RAA assay. Curves 1−10: nucleic acids of G. parasuis, S. suis, K. Pneumoniae, A. pleuropneumoniae, M. hyopneumoniae, S. aureus, P. multocida, S. typhimurium, E. coli, and negative control, respectively. (a) The specificity results of real-time RAA by real-time fluorescence readout. (b) The specificity results of RAA-VDA detection by a portable blue light imager with an excitation wavelength of 480 nm.</div>

Transboundary and Emerging Diseases

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Figure 4: Rapid Visual Detection of Glaesserella parasuis with a Real-Time Recombinase-Aided Amplification Assay