Rapid Visual Detection of Glaesserella parasuis with a Real-Time Recombinase-Aided Amplification Assay

Figure 5

The comparison of the sensitivity with G. parasuis RAA and qPCR assays. Curves 1−8 : 10⁶–10⁰ copies of APC2 and negative control, respectively. (a) The sensitivity results of qPCR assay. (b) Probit regression analysis of qPCR assay with the data of eight repeats. The detection limit at 95% reliability (67.17 copies/reaction) is marked by a triangle. (c) The sensitivity results of real-time RAA assay by real-time fluorescence readout. (d) Probit regression analysis of real-time RAA assay by real-time fluorescence readout with the data of eight repeats. The detection limit at 95% reliability (67.17 copies/reaction) is marked by a triangle. (e) The sensitivity results of RAA-VDA by a portable blue light imager with an excitation wavelength of 480 nm. (f) Probit regression analysis of RAA-VDA detection with the data of eight repeats. The detection limit at 95% reliability (143.43 copies/reaction) is marked by a triangle.

(a)

(b)

(c)

(d)

(e)

(f)