Rapid Visual Detection of Glaesserella parasuis with a Real-Time Recombinase-Aided Amplification Assay

Table 2

Repeatability and reproducibility analysis of the real-time RAA assay.

Concentration (copies/reaction)	Repeatability (intra-assay)				Reproducibility (interassay)
	Mean	SD	CV (%)		Mean	SD	CV (%)
High (10^6)	183	3.00	1.64		182	6.93	3.81
Medium (10^4)	268	9.17	3.42		272	18.33	6.74
Low (10^2)	644	27.71	4.30		628	36.66	5.84

Mean, the average of threshold times (second) of three independent real-time RAA reactions; SD, standard deviation; CV, coefficient of variation.