First Report of Porcine Parvovirus 2 (PPV2) in Pigs from Colombia Associated with Porcine Reproductive Failure (PRF) and Porcine Respiratory Disease Complex (PRDC)

Table 1

Set of primers designed for amplification of PPV2 genome from nt 380 to 4333.

Primer	Sequence	Product length (bp)	GenBank position/accession number
PPV2 S1-Ry	CCGACAGGATAAGTGTCGAG	1,246	151-1397/MW853943^a
PPV2 S1-Q2	GTTTGCCCTTAATGTCGATCC
PPV2 S2F	CCTCTCAATCCCTGATTTGG	1,137	1340-2477/MW853943^a
PPV2 S2R	GTAAAGCTGTAACAGAGCCTGCC
PPV2 S3F	GGATCAATGCCCTTCTGAGG	973	2361-3334/MW853943^a
PPV2 S3R	GGTAAGACTTTGTCCAGCTCC
PPV2 S4F	GGACACGTTAGCGGATCTCAT	1,003	3340-4343/MW853943^a
PPV2 S4R	GGTTATGACGGAGGTTCAACG
PPV2-S5-Cnvirus	TTACGAGTTTCCCAGTCTCG	1,150	4273-5423/MW853943^a
PPV2-S5-Ry	CCGACAGGATAAGTGTCGAG

^aGenBank reference sequence. Primers PPV2 S1 and S5 (Ry, Q2, and Cnvirus) were obtained from [5]; primers PPV2 S2, PPV2 S3, and PPV2 S4 were designed.