The VP1/2 Protein of a New Recombinant PRV Strain Promotes the Infectivity and Pathogenicity of PRV in Northeastern China

Figure 1

Isolation, identification, and culture of CH/HLJPRVJ/2023. (a) Detect PRV positive results in the cerebellar, controls were negative. (b) The supernatant of the 20^th passage of infected Vero cells was collected for transmission electron microscopy (TEM), and the bilayer structure of PRV particles was observed. (c) In-depth results of viral genome sequencing. (d) CH/HLJPRVJ/2023 genome map, including GC content, CDS region, and blast to Bartha-K61. (e) gD gene cloning plasmid was constructed to prepare PRV standard curve (n = 3). (f) Organization grinding fluid as P0 generation, to use its infection Vero cell on the freeze–thaw centrifugal clear for P1, in turn, get to P20 generation, detecting the three generations of copy number of the virus (n = 3, , , and ). CH/HLJPRVJ/2023 was blindly passaged to P8 generation to develop a cytopathic effect, and the TCID₅₀ of P8 and P20 generations was detected (n = 3, ).

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