Complete Genome Sequence and Construction of an Infectious Bacterial Artificial Chromosome Clone of a Virulent Duck Enteritis Virus Strain XJ

Figure 3

Construction of recombinant virus XJ BAC. (a) Schematic drawing of the cloning procedure to introduce the pHA2 vector into the DEV XJ genome. (b) Homogeneous population of recombinant virus rDEV XJ in DEFs. (c) Restriction fragment analysis of full-length BAC clone pDEV-XJ. The left is the prediction based on the complete DEV XJ genome by using Vector NTI. (d) Rescue of the recombinant virus XJ BAC. Plasmids from a positive colony were transfected into DEFs by calcium phosphate, and with continuous observation, the XJ BAC fluorescent marker protein EGFP was expressed in DEFs.

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