Complete Genome Sequence and Construction of an Infectious Bacterial Artificial Chromosome Clone of a Virulent Duck Enteritis Virus Strain XJ

Figure 6

Construction and identification of recombinant viruses. (a) Schematic representation of the two-step RED-mediated recombination process for constructing the US3-deleted mutant and LORF3-deleted mutant. (b) PCR identification of the two-step RED-mediated recombination for the US3-deleted mutant. (c) PCR identification of two-step RED-mediated recombination for the LORF3-deleted mutant. (d) Rescue of recombinant viruses.

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