Complete Genome Sequence and Construction of an Infectious Bacterial Artificial Chromosome Clone of a Virulent Duck Enteritis Virus Strain XJ

Figure 7

Comparison of biological characteristics of XJ-ΔUS3, XJ-ΔLORF3, and WT in vitro. (a) Multistep growth kinetics of XJ-ΔUS3, XJ-ΔLORF3, and WT. Viral titers of infected cells were assessed at different time points after inoculation with approximately 0.02 MOI of viruses. Statistical analysis of the differences in virus titers in the total, cell, and supernatant at each time point. Multistep growth curves were generated from three independent experiments. (b) Plaque morphology of XJ-ΔUS3, XJ-ΔLORF3, and WT. (c) Quantification of viral plaque size using Image J. Representative plaques from each sample (50 plaques per sample) were measured to quantify the results (ns, ; , ; , ; , ; and , ).

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