Epidemiological and Molecular Characteristics of Piroplasmids and Anaplasma spp. in Tan Sheep, Ningxia, Northwest China

Table 1

Primer sequences.

Name	Primers (5′−3′)	Annealing temperature (°C)	PCR products (bp)	References
T. luwenshuni18S-F	CATCTTCTTTTTGATGAGTTGA	57	1,123	Nijhof et al. [23]
T. luwenshuni18S-R	GCTTGATCCTTCTGCAGGTTCA
T. uilenbergi18S-F	AAGTGAATAGCGAGATGG	55	1,619
T. uilenbergi18S-R	TTAGGGAAAGTAAAGGTG
T. ovis18S-F	TTTTGCTCCTTTACGAGTCTTT	52	904
T. ovis18S-R	TCGTTCACGATTAATAATTGCA
B. motasi18S-F	TTTGCGATGTTCCATTCA	52	1,489	This study
B. motasi18S-R	CACCTACGGAAACCTTGT
B. ovis18S-F	TGATCCTGCCAGTAGTCAT	62	1,650
B. ovis18S-R	CACCTACGGAAACCTTGT
A. phagocytophilum16S-F	TGGGATAGCCACTAGAAAT	55	1,297	Barlough et al. [24]
A. phagocytophilum16S-R	GTACAAGACCCGAGAACG
A. bovis16S-F	CTCGTAGCTTGCTATGAGAAC	55	551
A. bovis16S-R	TCTCCCGGACTCCAGTCTG
A. ovis msp4-F	GGGAGCTCCTATGAATTACAGAGAATTGTTTAC	54	870	De La Fuente et al. [25]
A. ovis msp4-R	CCGGATCCTTAGCTGAACAGGAATCTTGC