Outbreaks of Elizabethkingia miricola Caused Fatal Meningitis-Like Disease in Cultured Bullfrogs

Figure 3

Gram staining, culture, and virulence factor detection of isolated bacteria. (a) Gram stain. (b) Growth in the LB medium at 30°C for 24 hr (left); growth in the TSA medium at 30°C for 24 hr (right). (c) Virulence factor detection (M: DM2000 plus; 1: fabG; 2: fabV; 3: wecB; 4: ureB; 5: aceA; 6: acyl; 7: Hly; 8: wbpO). (d) On the blood medium, cultured at 37°C for 24 hr (left); the same medium was cultured at 30°C for 24 hr (right). (e) Production of the hemolytic ring in isolated bacteria.

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