Outbreaks of Elizabethkingia miricola Caused Fatal Meningitis-Like Disease in Cultured Bullfrogs

Table 1

Primers for detection of E. miricola virulence genes.

Virulence genes	Primer sequence (5^′-3^′)	Tm	Product size (bp)
fabG	ATGAAACTATTAGAAGGAAAAGTAG	51	744
	CTAAGTTAACATTCCGCCA
fabV	ATGATCATACAACCACGTGTTA	53	1,203
	TTATCCTTCTATACTTGGGATGT
wecB	ATGAAGAAACTAAAAGTAATGACG	53	1,140
	TTAAATTTCTTCAGACCAGACAG
ureB	ATGATACCAGGAGAAATTTTTGT	54	369
	TTACAGGTTTTTAAAATTTAATTGA
aceA	ATGAAAACTATTCAGGAACTACAAC	54	1,275
	TTAGAATTGTGCTGTTTCTGTAGA
acyl	ATGTCAGACATCGCATCAA	51	240
	TTATTTGTTGACTACTTCTTCAAT
hly	ATGGGCAGGACAGTTGTC	52	174
	TTATTTTCCTAGTTCTAATTGATT
wbpO	ATGAACAATCACCGAATAGC	53	1,293
	TTATAATCTTGCATCTACGATCTC