Palm Multidiagnostic of <i>Mycoplasma pneumoniae</i>, <i>Chlamydia pneumoniae</i>, <i>Haemophilus influenzae</i>, and <i>Streptococcus pneumoniae</i> Using One-Tube CRISPR/Cas12a

<div>Construction of the RPA-Cas12a-based method for multiplex detection of sputum pathogens. Step 1, sputum samples were collected from patients. Step 2, the Sputasol method treats the sputum samples to release DNA. Step 3, one-tube method detection. Step 4, test results output. The one-tube assay includes RPA and CRISPR reaction components, where RPA can simultaneously amplify four DNA targets, specifically Mp, Cp, Hi, and SP. The Palm assay device, PaCD, integrates reaction heating and result detection, enriching POCT options.</div>

Transboundary and Emerging Diseases

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Figure 1: Palm Multidiagnostic of <i>Mycoplasma pneumoniae</i>, <i>Chlamydia pneumoniae</i>, <i>Haemophilus influenzae</i>, and <i>Streptococcus pneumoniae</i> Using One-Tube CRISPR/Cas12a 

Figure 1 | Palm Multidiagnostic of Mycoplasma pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, and Streptococcus pneumoniae Using One-Tube CRISPR/Cas12a 