Palm Multidiagnostic of Mycoplasma pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, and Streptococcus pneumoniae Using One-Tube CRISPR/Cas12a

<div>Screening of crRNA. (a) Relative position of crRNAs on target genes. (b–e) Specificity and efficiency screening of crRNAs. (f) Relative values of specificity and efficiency in heat map analysis of crRNAs. (g, h) One-tube fluorescence detection and statistics of optimal crRNA. (i) Nucleic acid gel validation of target genes. Marker, DL2000. (j) Sequence of Mp, Cp, Hi, and SP optimal crRNAs. NTC, no template control.</div>

Transboundary and Emerging Diseases

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Figure 2: Palm Multidiagnostic of Mycoplasma pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, and Streptococcus pneumoniae Using One-Tube CRISPR/Cas12a

Figure 2 | Palm Multidiagnostic of Mycoplasma pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, and Streptococcus pneumoniae Using One-Tube CRISPR/Cas12a 