Research Article

Palm Multidiagnostic of Mycoplasma pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, and Streptococcus pneumoniae Using One-Tube CRISPR/Cas12a

Figure 3

Screening of the RPA primers. (a) Relative position of RPA primers and screening of optimal primers. (b) One-tube reactions corresponding to different RPA primer combinations. (c–f) Statistics of fluorescence intensity of different RPA primer combinations corresponding to a one-tube reaction. (g–j) Heat map method for screening the optimal primer combinations. NTC, no template control.
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