Palm Multidiagnostic of Mycoplasma pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, and Streptococcus pneumoniae Using One-Tube CRISPR/Cas12a

Figure 4

Sensitivity analysis of Cas12a-based fluorescent method of four respiratory pathogens. (a–d) Detection sensitivity of Mp, Cp, Hi, and SP in plasmid samples, respectively. (e–h) Detection sensitivity of Mp, Cp, Hi, and SP in sputum samples, respectively. Three replicates of the assay were performed to validate the LOD of the PaCD method for the plasmid and sputum samples. represent statistically significant differences between groups ().

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