Isolation and Pathogenicity of a Chinese Porcine Astrovirus Type 5 Strain HNPDS-01 and Its Influence on Cecum Microbiota in Piglets

Figure 1

Isolation and identification of PAstV 5-HNPDS-01 strain. (a) The CPE of 544LLC-PK infected with PAstV 5-HNPDS-01 at 24, and 48 hpi, the scale bar is 100 μm. (b) TEM of PAstV 5-HNPDS-01 particles, sample was negatively stained with 2% (v/v) phosphotungstic acid, the scale bar is 50 nm. (c) Plaque-purified PAstV 5-HNPDS-01547 strain. (d–i) LLC-PK cells, ST cells, and IPEC-J2 cells were infected with PAstV 5-HNPDS-01 at an MOI of 0.02 and monitored for CPE. Collecting the samples at 6, 12, and 24 hpi was performed to IF and WB (PAstV 5 capside-protein antibody prepared in our lab). The scale bar is 200 μm.

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