Isolation and Pathogenicity of a Chinese Porcine Astrovirus Type 5 Strain HNPDS-01 and Its Influence on Cecum Microbiota in Piglets

Figure 3

Complete genome sequence and phylogenetic analysis of PAstV 5-HNPDS-01. (a) Complete genome sequence of PAstV 5-HNPDS-01 was amplified by RT-PCR with full-sequence amplification primers (Table 1). (b) The whole genome sequence was assembled by seqMan the phylogenetic tree was subsequently constructed from the aligned sequences using the neighbor-joining method (bootstrap was 1,000 replicates) of MEGA X software. Red triangles indicated the PAstV 5-HNPDS-01. Phylogenetic tree for the ORF 2 gene was constructed with above method, of which red triangles indicate the ORF 2 of PAstV 5-HNPDS-01.

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